全文获取类型
收费全文 | 1080297篇 |
免费 | 122299篇 |
国内免费 | 684篇 |
出版年
2018年 | 9899篇 |
2017年 | 9466篇 |
2016年 | 13449篇 |
2015年 | 18664篇 |
2014年 | 21790篇 |
2013年 | 31132篇 |
2012年 | 34647篇 |
2011年 | 35284篇 |
2010年 | 24002篇 |
2009年 | 22009篇 |
2008年 | 31130篇 |
2007年 | 32033篇 |
2006年 | 30123篇 |
2005年 | 29056篇 |
2004年 | 28768篇 |
2003年 | 27817篇 |
2002年 | 26815篇 |
2001年 | 48969篇 |
2000年 | 49239篇 |
1999年 | 39049篇 |
1998年 | 13796篇 |
1997年 | 14486篇 |
1996年 | 13620篇 |
1995年 | 13159篇 |
1994年 | 12819篇 |
1993年 | 12843篇 |
1992年 | 32632篇 |
1991年 | 31846篇 |
1990年 | 30992篇 |
1989年 | 30330篇 |
1988年 | 28170篇 |
1987年 | 26638篇 |
1986年 | 24805篇 |
1985年 | 24682篇 |
1984年 | 20622篇 |
1983年 | 17498篇 |
1982年 | 13449篇 |
1981年 | 12024篇 |
1980年 | 11366篇 |
1979年 | 19116篇 |
1978年 | 15026篇 |
1977年 | 13653篇 |
1976年 | 12626篇 |
1975年 | 13938篇 |
1974年 | 14786篇 |
1973年 | 14719篇 |
1972年 | 13226篇 |
1971年 | 12038篇 |
1970年 | 10286篇 |
1969年 | 9966篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
S Chwetzoff S Tsunasawa F Sakiyama A Ménez 《The Journal of biological chemistry》1989,264(22):13289-13297
The venoms of the Naja species are known to be cytotoxic. This toxicity has been attributed to the presence of small nonenzymatic polypeptides of 60 amino acid residues, designated as cardiotoxins or cytotoxins. We investigated the cytotoxic potency of Naja nigricollis venom fractions and isolated another type of cytotoxic component which is even more potent than cardiotoxins. This cytotoxic compound, which was designated as nigexine, was purified to homogeneity and its amino acid sequence was determined. Nigexine is a basic phospholipase A2 consisting of a single chain of 118 amino acids. A detailed investigation of the cytotoxic effects on epithelial FL cells, C-13T neuroblastoma cells, and promyelocytic leukemia HL 60 cells revealed that nigexine not only altered cell viability but also prevented cell proliferation. This is a property that was specific to nigexine since other phospholipases A2 from various sources had no detectable cytotoxic activity. The cytotoxic activity of nigexine was not dependent on the presence of divalent cations, unlike its enzymatic activity. In particular, the cytotoxic activity of nigexine was identical in the presence or absence of either 2 mM Ca2+ or Sr2+, or 6 mM EDTA. We also present evidence based on chemical modifications that cytotoxic activity was not correlated with enzymatic activity. Thus, modification with parabromophenacyl bromide totally abolished the enzymatic activity of nigexine, which nevertheless retained 6-20% of the cytotoxicity of native nigexine. Conversely, treatment with cyanogen bromide gave a compound that retained 7% of the enzymatic activity of the parent molecule but was devoid of detectable cytotoxicity. 相似文献
103.
104.
Omar Díaz-Segura Jordan Golubov María C. Mandujano José Alejandro Zavala-Hurtado 《Plant Species Biology》2020,35(4):270-282
Reproductive systems are life attributes important in defining the demography and genetic constitution of invasive alien species populations. We describe the phenology, floral behavior and floral visitors in Mexican populations of Leonotis nepetifolia considered invasive in America, Asia and Oceania. The mating system was determined through pollination experiments and, with a morphological analysis of flowers (outcrossing index, OCI) and pollen/ovule ratio, the breeding system was evaluated. Germination of 1 and 2-year-old seeds was tested to assess the potential characteristics of germination. Leonotis nepetifolia was reproductive for 7 months (June to December) and tended towards a specific season during autumn. Anthesis lasted 36 hr with protogyny and no hercogamy, with floral visitors of Apodiformes, Hymenoptera, Lepidoptera and Thysanoptera. Pollination experiments indicated a mixed mating system, whereas the OCI and the pollen/ovule ratio pointed towards a facultative xenogamous breeding system. Seed production was high (1,445 ± 132 seeds/plant); the seeds had potential longevity and were neutral photoblastic. One-year-old seeds germinated slightly later ( = 2.6 ± 0.11 days) than 2-year-old seeds ( = 1.9 ± 0.02 days), both synchronously (IS1yr = 0.88 ± 0.03 and IS2yr = 0.82 ± 0.02). Germination percentage for 1-year-old seeds was lower (55.33 ± 4.40%) than that of 2-year-old seeds (94.18 ± 0.59%), suggesting a potential longevity of the seeds in an optimal environment. Reproductive characteristics, such as wide reproductive period, mixed breeding system, copious seed production, seeds with potential longevity, and quick and synchronic germination in different light conditions, favor the invasive capacity of Leonotis nepetifolia. 相似文献
105.
M. V. Bazunova L. A. Sharafutdinova R. Yu. Lazdin V. V. Chernova D. N. Mixonov V. P. Zakharov 《Applied Biochemistry and Microbiology》2018,54(5):474-477
The conditions to obtain materials with elastic-viscous properties based on chitosan succinamide have been studied. A decreased polymer content and a transition from visco-elastic liquids to elastic-viscous systems were shown upon the addition of glycerol to an aqueous solution of chitosan succinamide. The systemic response, biological compatibility, and dynamics of bioresorbability of the obtained materials were studied during implantation in laboratory animals. 相似文献
106.
107.
Continental‐scale macrofungal assemblage patterns correlate with climate,soil carbon and nitrogen deposition 下载免费PDF全文
108.
109.
Y.-M. D. 《Option/Bio》2018,29(573-574):14
110.
Plasma Physics Reports - So far, the detailed experimental effect of the inductance on the X-ray yield in the Filippov-type plasma focus devices has not been documented in literature. In this... 相似文献